法医DNA快速检验研究进展

摘要

Forensic DNA typing technology has become a hot topic, mainly used in individual identification and paternity tests. This paper fully explains and illustrates the experiment principle about the forensic DNA test rapid research progress including rapid extraction,rapid amplification, rapid equipment, micro-fluidic chip technology and full integration. The author not only suggests that using strong suppression of buffer system, high efficiency enzyme and simultaneous rapid amplification can achieve rapid PCR detection technology but also puts forward the realization of chip DNA detection. Therefore the domestic portable automatic detection equipment is the main direction of the future development.%法医DNA快速检验技术是目前研究的热点,主要应用于个体识别和亲子鉴定领域.该文分别从快速提取,快速扩增,快速设备,微流控芯片,全自动DNA分型检测设备方面对现有DNA快速检验的技术方法,实验原理进行充分说明和举例论证,同时对该行业在快速检验方法的选择问题上提出明确的建议——采用强抑制力的缓冲体系,高效酶并结合快速扩增程序实现快速PCR检测技术,同时提出芯片化DNA检测的实现方式,指出国产的便携式全自动快速检测仪器是未来发展的主要方向.