香菜挥发油体外抑制Saos-2细胞生长与迁移

摘要

Objective To extract coriander volatile oil and investigate its effects on growth and migration of human Saos-2 cells . Methods Ultrasound-assisted ethanol extraction was used to prepare coriander volatile oil. MTT assay was used to determine the growth inhibion of Saos-2 cells by volatile oil. Cell migra-tion was examined using Boyden chamber assay. Expression of 21 1/ 1, 27 1, 23 1 and 100 4 were examined by real-time PCR. Results The results showed that the yield of coriander volatile oil was 0.424% using ultrasound-assisted ethanol extraction. Coriander volatile oil inhibited the growth and migra-tion of Saos-2 cells, along with the decrease in the expression levels of 21 1/ 1, 27 1 and 100 4. Conclusions Coriander volatile oil can inhibit Saos-2 cell growth and migration in vitro, which may involve 21 1/ 1, 27 1 and 100 4.%目的：探讨香菜挥发油对Saos-2肿瘤细胞株体外生长及迁移的影响。方法采用超声波辅助醇提法制备香菜挥发油；MTT法检测香菜挥发油对Saos-2细胞生长抑制作用；Transwell趋化小室检测细胞迁移抑制作用；定量PCR检测细胞内211/1、271、231和1004的表达变化。结果使用超声辅助醇提法，香菜挥发油得率为0.424%；香菜挥发油可明显抑制Saos-2细胞的生长与迁移，伴随细胞内211/1及271的升高及1004降低，显示具有较强的抗成骨肉瘤活性。结论香菜挥发油可体外抑制Saos-2细胞生长及迁移，其可能机制与211/1、271及1004有关。