Matrix Interference in Serum Total Thyroxin （T₄） Time-resolved Fluorescence Immunoassay （TRFIA） and Its Elimination With the Use of Streptavidin-biotin Separation Technique

摘要

In development of serum total thyroxin TRFIA using the surface second-antibody (S-Ab) as separation agent, a significant bias of measurement caused by matrix interference when the surface S-Ab shows a relatively low binding capacity for primary anti-T4 monoclonal antibody (McAb) is studied. The bias ranges from 10% to 78%, depending on the matrix of individual samples and the binding capacity of the surface S-Ab prepared. So, a new separation system based on the use of a highly active surface streptavidin and biotinylated anti-T4 McAb is employed. The results indicate