To explore the bioavailability of DON to toxic algae and its chemical nature in Xiamen Tingxi reservoir, an experiment on the growth of Microcystis aeruginosa was conducted based on the solid phase extraction and membrane separation technique for water sample. Results showed that it is variable for the different seasonal concentrations of DON in Tingxi reservoir. DON concentration was higher in dry season, but lower in wet season. The primary components of DON were hydrophobic and >3kDa counterparts in both dry and wet season. The bioavailable DON reached 65.20% in the typical reservoir. Specifically, the hydrophilic and <3kDa counterparts of DON were relatively easy to be utilized by Microcystis aeruginosa. As the security of source water and the protection of water environment are concerned, it is of great importance to firstly remove the hydrophilic and low molecular weight counterparts of DON in freshwater.%为探究厦门汀溪水库溶解有机氮(DON)对有毒藻种的生物有效性及其化学本质,采用水样分子质量和极性分级技术,进行铜绿微囊藻(Microcystis aeruginosa)培养实验.结果表明,汀溪水库中DON含量呈季节性差异,在枯水期比较高,而丰水期比较低.汀溪水库DON在不同季节中的主要成分都是疏水组分和＞3kDa组分.汀溪水库DON的生物有效性为65.20％较疏水组分和＞3kDa组分而言,亲水组分和≤3kDa组分DON更易被铜绿微囊藻利用.从水环境和水源保护角度出发,应首先考虑消除DON中的亲水性组分和小分子质量组分.
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