从胶州湾海底沉积物中分离筛选出一株异养硝化-好氧反硝化菌株y6,通过菌株y6的形态以及生理生化特性和16S rRNA基因序列的分析,鉴定该菌株属于克雷伯氏菌属(Klebsiella sp.).在不同的环境条件下,测定菌株y6的生长情况和脱氮能力,研究其同步脱氮除碳特性.实验结果表明,菌株y6的最佳碳源为柠檬酸三钠,最适宜pH值为7.0,最适合的C/N为17.菌株y6在以NH4Cl、KNO3和NaNO2为唯一氮源的反应系统中均有较好的脱氮效果,去除率分别为99.67%、100%、99.20%.菌株y6在脱氮的同时能高效地去除有机物,COD的去除率分别为82.17%、95.75%和97.83%.菌株y6在硝化过程中没有亚硝态氮和硝态氮的积累.在按不同比例混合氮源的反应系统内,首先进行的是硝态氮的好氧反硝化,随后进行的是氨氮、亚硝态氮和COD的去除.在有亚硝态氮存在时氨氮的去除率略低,亚硝态氮会影响y6的异养硝化过程,异养硝化对好氧反硝化过程没有影响.%A heterotrophic nitrification-aerobic denitrification strain, named y6, was isolated from sediment of Jiaozhou Bay. It was identified asKlebsiellasp. based on the morphological, physiological and analysis of 16S rRNA gene sequence. Under different environmental conditions, measured strain y6’s growth situation and denitrification ability, so as to analyze its characteristics of simultaneous removal of nitrogen and carbon. The results showed that the optimal carbon source was sodium citrate; the optimal pH was 7.0; the optimal C/N was 17. In the reaction system of NH4Cl, KNO3 and NaNO2 for the only nitrogen source, Strain y6had better denitrification effect. The removal efficiencies were 99.67%, 100% and 99.20%. Strain y6could efficiently remove organic matter simultaneously in the process of denitrification, with COD removal rate of 82.17%, 95.75% and 97.83%, respectively. Almost no nitrate nitrogen and nitrite nitrogen accumulation in the y6 heterotrophic nitrification process. In the different mixing ratio of reaction system, the first were aerobic denitrification of nitrate nitrogen, Subsequent were ammonia nitrogen, nitrite nitrogen and COD removal. Ammonia nitrogen removal rate was only slightly lower in the presence of the nitrate nitrogen. The nitrite nitrogen affected y6heterotrophic nitrification process. The heterotrophic nitrification of y6had no effect on aerobic denitrification.
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