低温条件(10℃)下,采用定时定量转接、间歇式逐步提高PAHs浓度的方法,从天津滨海湿地石油污染土壤中获得能以多环芳烃芘为唯一碳源和能源生长的菌群H和纯化菌株DYC-1,经生理生化和16S rDNA基因序列BLAST对比结果分析,菌株DYC-1属于红球菌属(Rhodococcus)。降解特性分析结果表明,10℃低温条件下,红球菌DYC-1与菌群H降解能力相似,对20mg/L芘的15d降解率达到35%以上；红球菌DYC-1具有较好的耐盐能力和较广的降解底物谱,菌株DYC-1的最优降解条件为：低温10℃,盐度2%的条件下,在pH8,摇床转速为110r/min,接菌量为5%时,对于初始浓度为20mg/L的芘能达到35%以上的降解率。%The community H and single-strainDYC-1 was isolated by timing quantitative culture and domestication method of adding the concentration of PAHs gradatim from Tianjin coastal wetland petroleum contaminated soil under the low temperature condition. The community H and single-strainDYC-1used pyrene as solo carbon source for growth. The bacteria were identified asRhodococcussp. based on the BLAST sequence analysis of 16s rDNA and its morphological and physio-biochemcial characteristics. Analysis results showed that the degrading ability of single-strain DYC-1 was similar to community H under the low temperature condition. The degradation rate of them was above 35% at high concentrations of pyrene. The single-strain DYC-1 had more salt-resisting ability and wide substrate degradation ability. The optimal degradation conditions of single-strain DYC-1 was 2% salinity, pH8, 10℃, 110r/min rotate speed and 5% capacity of bacteria. The degradation rate of pyrene was high with 20mg/L initial concentration in the optimal degradation conditions.
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