Determination of Glycyrrhizic Acid Dipotassium in Wash and Care Cosmetics by HPLC

摘要

To establish a method for the determination of glycyrrhizic acid dipotassium in various cosmetics(shampoo,shower gel,hair conditioner and cleanser)with High Performance Liquid Chromatography.ZORBAX SB-C18 column(4.6 mm×250 mm,5μm)was used.The 0.01 mol/L phosphoric acid solution-acetonitrile(63:37)was used as the flow equivalent elution.The sample was extracted by ultrasonic methanol and detected by diode array detector,The extraction solvent and chromatographic conditions were studied and optimized.Glycyrrhizic acid dipotassium has a good linear relationship in the concentration range of 1~200μg/mL(R²=0.9997).The average recovery rates of glycyrrhizic acid dipotassium in shampoo,shower gel,hair conditioner and cleanser were 92.92%~101.69%.The method was precise and accurate and could be used for the qualitative and quantitative analysis of Glycyrrhizic acid dipotassium in cosmetics.