FLUORIMETRIC DETERMINATION OF TETRACYCLINE IN SERUM AND URINE

摘要

The Eu-Tetracycline（TC）-TOPO-sodium dodecyl sulfonate（SDS）system wasstudied,Experiments showed that the maximum fluorescence intensity was obtainedin the pH range of 7.5-8.7 and the concentrations of Eua+,TOPO and SDS are1.0×10-6mol/L,1.0×10-8mol/L and 1.0×10-8mol/L,respectively,This fluorescencesystem can be used for the determination of TC in serum and urine,Beer’s Law isobeyed in the range of 2.0×10-8mol/L=1.0×10-6mol/L for the concentration of TC.The determination limit is 1.2×10-8mol/L,The composition and the luminescencemechanism were discussed.