棘白霉素类化合物脱酰基酶产生菌的转化工艺研究

摘要

In this paper,comprehensive study on transformation process of deacylase-producing strain ZH-020 for echinocandin compounds has been conducted. The optimum transformation conditions are obtained as follows:substrate concentration is 1200mg·L-1,transformation time is 24 h and transformation temperature is 30 ℃. Uniform design method is used to optimize the fermentation medium and the optimum medium contains 2.5% groundnut meal, 1.3% glucose, 1.0% sucrose, 0.1% KH2PO4 and 0.05% MgSO4. For the optimized process,the transformation rate is 33. 29% higher than that for the control.%对棘白霉素类化合物脱酰基酶产生菌ZH-020的转化工艺进行了研究.确定最佳转化条件为:底物浓度1200mg·L-1、转化时间24 h、转化温度30℃；通过均匀设计实验确定优化的发酵培养基配方为:花生饼粉2.5％、葡萄糖1.3％、蔗糖1.0％、磷酸二氢钾0.1％、无水硫酸镁0.05％；在优化条件下,转化率比对照提高了33.29％.