雷公藤内生真菌Pestalotiopsis disseminatar次级代谢产物研究

摘要

采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱及PHPLC等手段对雷公藤(Tripterygiumwilfordii Hook.f.)内生真菌广布拟盘多毛孢(Pestalotiopsis disseminata)的发酵次级代谢产物进行分离纯化,通过理化性质及波谱学数据分析对化合物结构进行鉴定.结果表明,从内生真菌Pestalotiopsis disseminata的次级代谢产物中共分离并鉴定了7个单体化合物,分别为7-羟基-5-甲氧基-4,6-二甲基苯酞(Ⅰ)、腺苷(Ⅱ)、尿苷(Ⅲ)、邻苯二甲酸二正丁酯(Ⅳ)、邻苯二甲酸二异丁酯(Ⅴ)、1,1′-二(1,1′-羧基乙基)醚(Ⅵ)、赤藓醇(Ⅶ),其中化合物Ⅱ、Ⅲ、Ⅵ、Ⅶ为首次从该属真菌中分离得到,化合物Ⅰ、Ⅴ为首次从该种真菌中分离得到.%The secondary metabolites of Pestalotiopsis disseminata,a endophytic fungus isolated from Tripterygiumwilfordii Hook.f.,were isolated and purified through silica gel and Sephadex LH-20 gel column chromatography as well as PHPLC method,and the structures of obtained compounds were identified by physi-cochemical properties and spectral data.Results showed that,seven compounds which isolated from the seconda-ry metabolites of Pestalotiopsis disseminata were identified as 7-hydroxy-5-methoxy-4,6-dimethylphthalide (Ⅰ),adenosine(Ⅱ),uridine(Ⅲ),dibutyl phthalate(Ⅳ),diisobutyl phthalate(Ⅴ),1 ,1′-bis(1 ,1′-carboxyethyl) ether(Ⅵ),erthyritol(Ⅶ),respectively.Compounds Ⅱ,Ⅲ,Ⅵ and Ⅶ were firstly obtained from Pestalotiopsis genus,and compounds Ⅰ and Ⅴ were firstly found from fungus Pestalotiopsis disseminata.