端粒酶活性检测方法研究进展

摘要

Human telomerase is a ribonucleoprotein complex,which functions as a telomere terminal transferase by catalytic adding telomere repeat TTAGGG to the end of chromosome using its RNA as the template. Due to a very strong association between telomerase activity and malignancy in nearly all types of cancer, telomerase plays a key role in the tumor occurrence as well as development. Monitoring telomerase activity is believed to be important for cancer diagnosis, prediction and provides an important basis for clinical treatment. This review summarizes recent development for detection of telomerase activity, with emphasis placed on in situ detection.%人体端粒酶是一种核糖核蛋白复合物，在生物体内，以其自身的RNA为模板，通过催化添加端粒重复序列TTAGGG至染色体末端。由于在绝大多数癌症中端粒酶活性与恶性肿瘤之间存在着较大的关联，其在肿瘤的发生、发展过程中起着重要作用，因而对端粒酶活性的检测可为癌症的诊断、预测以及临床治疗提供重要依据。该文总结了近几年来针对端粒酶活性几种不同的检测方法，重点强调了几种机体内原位检测方法，并适当的进行了分析比较。