Affinity Adsorption of Bilirubin on Cibacron Blue F3GA-modified Microporous Polytetrafluoroethylene Capillary

摘要

Bilirubin removal from human plasma was obtained via an affinity microporous polytetrafluoroethylene(MPTFE) capillary.The new adsorbent comprised grafted glycidyl methacrylate(GMA) via radiation-induced polymerization as hydrophilic coating and reactive sites;ethylenediamine(EDA) as a spacer arm;Cibacron Blue F3GA(CB F3GA) as an affinity ligand;MPTFE capillary as the supporting matrix.The average density of CB F3GA attachment to MPTFE capillaries was found to be 136.5 μmol/g.The capacity of bilirubin adsorbed on affinity MPTFE capillaries is 76.1 mg bilirubin/g polymer(at 25°C).This new adsorbent has advantages over both membrane and traditional micro-column,and this system is stable and easy to operate.The results of blood tests suggest the CB F3GA affinity capillary has good blood compatibility.