Ionic Liquid-based Microwave-assisted Liquid-liquid Microextraction and High Performance Liquid Chromatography Determination of Sulfonamides from Animal Oils

摘要

The authors performed ionic liquid-based microwave-assisted liquid-liquid microextraction(IL-based MALLME) coupled with high performance liquid chromatographic separation for the determination of 6 sulfonamides (SAs) from animal oils.The target analytes were extracted from animal oil samples with sodium hydroxide solution containing 1-butyl-3-methylimidazolium tetrafluoroborateand as the extraction solvent under microwave irradiation.The experimental parameters of the IL-based MALLME,including types of ILs,volume of IL,amount of ion-pairing agent(NH4PF6),pH value of sample solution,and extraction temperature and time were evaluated.The limits of detection and quantification obtained were in a range of 0.4-0.5 μg/kg and a range of 1.2-1.8 μg/kg,respectively.The accuracy of the method was evaluated by analyzing five spiked animal oil samples at two fortified levels(5 and 50 μg/kg),and the recoveries of SAs varied from 81.4％ to 114.5％ with relative standard deviations ranging from 0.8％ to 9.0％.