大孔树脂对麻黄细辛附子汤生物碱成分静态吸附及解吸附性能研究

摘要

To select a best type of macroporous resin for the isolation and purification of alkaloids from Mahuang Xixin Fuzi decoction, high performance liquid chromatography was used to determine the contents of hydrochloride ephedrine,pseudoephedrine hydrochloride,benzoylmesaconine,benzoylaconine,benzoylhypaconine. The performance of static adsorption and desorption of four kinds of macroporous resins (HPD–100,HPD–722,HPD–400,HPD–600) were investigated. The results showed that the macroporous resin of HPD–722 was better than others with a stable adsorption process,larger adsorption capacity and higher desorption rate (87.26%). HPD–722 macroporous resin can be used to enrich and purify the compounds of alkaloids from Mahuang Xixin Fuzi decoction.%采用高效液相色谱法(HPLC)测定麻黄细辛附子(MXF)汤复方水提液中盐酸麻黄碱、盐酸伪麻黄碱、苯甲酰新乌头原碱、苯甲酰乌头原碱、苯甲酰次乌头原碱含量，考察了4种大孔树脂(HPD–100，HPD–722，HPD–400， HPD–600型)对5种生物碱的静态吸附、解吸附性能，筛选出分离纯化麻黄细辛附子汤中生物碱的最佳树脂。结果显示，HPD–722型树脂对5种生物碱的吸附过程稳定且吸附量大，具有较高的解吸附率(87.26%)，优于其它3种树脂。HPD–722型大孔树脂适用于富集麻黄细辛附子汤中的生物碱类化合物。