TGF-β1 gene-modified,immature dendritic cells delay the development of inflammatory bowel disease by inducing CD4^(+)Foxp3^(+)regulatory T cells

摘要

Inflammatory bowel disease(IBD)is caused by an uncontrolled immune response in the intestinal lumen,leading to inflammation in genetically predisposed individuals.Immunotherapy may be a promising approach to the treatment of IBD.Here,we show that transforming growth factor-β1(TGF-β1)gene-modified immature dendritic cells(imDCs)could enhance the inhibitory function of imDCs and delay the progress of IBD induced by dextran sodium sulfate in mice.The results of fluorescence-activated cell sorter(FACS)demonstrated that this protective effect is mediated partially by inducing CD4^(+)Foxp3^(+)regulatory T cells(Tregs)in mesentery lymph nodes to control inflammation.In vitro experiments also supported this hypothesis.In conclusion,we provide evidence that TGF-b1-modified bone marrow-derived imDCs may have a therapeutic effect to IBD.