Analysis of Accumulating Clonotypes of T Cell in Joints of a Spontaneous Murine Model of Rheumatoid Arthritis

摘要

＜正＞ SKG mouse,as a model of spontaneous rheumatoid arthritis （RA） bred recent years,is similar to the patientswith RA.We analyzed the clonotypes of T cell infiltrating into joints of SKG mice in initial stage and late stageof RA by using reverse transcriptase-polymerase chain reaction （RT-PCR） and subsequent single-strandconformation polymorphism （SSCP）.The results indicated that the percentages of clonotypes TCR Vβ2 andVβ8.2 of T cell clonotypes increased obviously to 72.3% and 60.2%,respectively.Mice number with identicalTCR Vβ2 and Vβ8.2 clonotypes also clearly increased in late stage of disease to 100% and 75%,respectively.These results mean that T cells with TCR Vβ2 and Vβ8.2 clonotypes probably play an important role in RAprogression of SKG mouse.Sequencing of the extracted DNA verified that common bands on SSCP gel werederived from the same T cell clones among samples from different joints.The results we obtained implied thatRT-PCR/SSCP method was a sensitive and credible method for analyzing T cell clonotypes.When the T cells ofSKG mouse were adoptively transferred to a nude mouse,it was verified that the T cells infiltrating into jointswere related to morbid formation of RA.Cellular ＆ Molecular Immunology.2004;1（4）:300-303.