Salmeterol attenuates the inflammatory response in asthma and decreases the pro-inflammatory cytokine secretion of dendritic cells

摘要

Salmeterol is a long-acting β2-agonist that activates adenylate cyclase,causing long-lasting bronchodilation and has been used for many years to control asthma.However,little information is available about the immunoregulatory effects of salmeterol.We found that salmeterol decreases the production of pro-inflammatory cytokines in a model of allergen-challenged mice that expressed tumor-necrosis factor-alpha,interleukin-1 and interleukin-6.Dendritic cells (DCs) are antigen-presenting cells and act as sentinels in the airway.We found that salmeterol (10-5 mol/I) reduced the inflammation caused by lipopolysaccharide (0.1 μg/ml) in activated murine bone marrow-derived DCs.Moreover,western blots demonstrated that this protective effect was mediated partially by inhibiting signaling through the nuclear factor-kappa B (NF-κB),mitogen-activated protein kinase (MAPK) pathways and dramatically decreased levels of p-ERK.We suggest that salmeterol regulates the inflammation of allergen-induced asthma by modulating DCs.In conclusion,we provide evidence that DCs are the target immune cells responsible for the action of salmeterol against asthma.