Global Gene Expression Profiling in Interleukin-12-Induced Activation of CD8+ Cytotoxic T Lymphocytes against Mouse Mammary Carcinoma

摘要

＜正＞ Interleukin-12（IL-12） is a critical cytokine representing the link between the cellular and humoral branches ofhost immune defense apparatus.IL-12-induced cytotoxic lymphocyte（CTL） development is a centralmechanism in immune responses against intracellular infectious agents as well as malignant growth.However,the molecular basis of tumor-specific CTL responses mediated by IL-12 remains poorly defined.In this study,we addressed this issue in a comprehensive manner to probe into IL-12-induced anti-tumor responses by globalgene expression profiling of mRNA expression in CD8+ T cells in a transplantable syngeneic mouse mammarycarcinoma model treated or not with recombinant IL-12.A strong tumor regression was induced by the IL-12treatment.An introspection of differential gene expression at an early stage of the IL-12-initiated CTLactivation reveals interesting genes and molecular pathways that may account for the marked tumor regression,and is likely to provide a rich source of potential targets for further research and development of effectivetherapeutic modalities.Cellular ＆ Molecular Immunology.2004;1（5）:357-366.