Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation and genomic labeling

摘要

Dear Editor, The CRISPR-Cas9 system has recently been widely adopted in genome editing due to its simplicity [1-3].Nuclease-deficient mutant dCas9 protein can be fused to effector domains and the fusion proteins can be guided by sgRNAs to genomic sites to regulate gene expression or label chromosomes [4-10].However,only one type of effector is applied in most experiments due to the exclusive sgRNA:Cas9 pairing.Moreover,multimerization by directly fusing multiple copies of effectors with dCas9 protein to achieve sufficient effector activity is technically challenging.