Lidamycin Induces Apoptosis of B-Cell Lymphoma Cells and Inhibits Xenograft Growth in Nude Mice

摘要

OBJECTIVE To study the cytotoxicity of Lidamycin (LDM)and its induction of apoptosis in Raji and Daudi cells of B-celllymphoma, and the inhibition of growth of the lymphoma Rajixenograft in nude mice.METHODS MTT assay was used to observe the inhibition byLDM on the proliferation of the Raji and Daudi cells. AnnexinV-FITC/PI double-stain, in combination with flow cytometry(FCM), was used to determine the induction of apoptosis by LDMin Raji cells. The B-cell lymphoma Raji xenograft model in nudemice was set up to detect the in vivo antitumor activity of LDM.RESULTS LDM markedly inhibited the proliferation of theRaji and Daudi cells in vitro, with IC_(50) values of 7.13 × 10^(-11) mol/Land 2.91 × 10^(-10) mol/L, respectively. The apoptotic rates of Rajicells were respectively 77.98% and 67.63% at 0.5 nmol/L and 0.25nmol/L of LDM, indicating an obvious induction of apoptosis inRaji cells. LDM inhibited the formation and growth of humanB-cell lymphoma Raji xenograft in nude mice. The inhibitionrates of tumor growth were respectively 74.9% and 65.2% in LDMat dosage group of 0.05 mg/kg and 0.025 mg/kg, suggesting anapparent prolongation of survival time in the nude mouse bearinglymphoma.CONCLUSION LDM can effectively induce apoptosis of theB-cell lymphoma cells and inhibit the xenograft growth in nudemice.