Experimental repetitive mild traumatic brain injury induces deficits in trabecular bone microarchitecture and strength in mice

摘要

To evaluate the long-term consequence of repetitive mild traumatic brain injury(m TBI) on bone, m TBI was induced in 10-week-old female C57 BL/6 J mice using a weight drop model, once per day for 4 consecutive days at different drop heights(0.5, 1 and 1.5 m) and the skeletal phenotype was evaluated at different time points after the impact. In vivo micro-CT(μ-CT) analysis of the tibial metaphysis at 2, 8 and 12 weeks after the impact revealed a 5%–32% reduction in trabecular bone mass. Histomorphometric analyses showed a reduced bone formation rate in the secondary spongiosa of 1.5 m impacted mice at 12 weeks post impact. Apparent modulus(bone strength), was reduced by 30%(P o 0.05) at the proximal tibial metaphysis in the 1.5 m drop height group at 2 and 8 weeks post impact. Ex vivo μ-CT analysis of the fifth lumbar vertebra revealed a significant reduction in trabecular bone mass at 12 weeks of age in all three drop height groups. Serum levels of osteocalcin were decreased by 22%, 15%, and 19% in the 0.5, 1.0 and 1.5 m drop height groups, respectively,at 2 weeks post impact. Serum IGF-I levels were reduced by 18%–32% in m TBI mice compared to contro1 mice at 2 weeks post impact. Serum osteocalcin and IGF-I levels correlated with trabecular BV/TV(r^2= 0.14 and 0.16, P o 0.05). In conclusion, repetitive m TBI exerts significant negative effects on the trabecular bone microarchitecture and bone mechanical properties by influencing osteoblast function via reduced endocrine IGF-I actions.