首页> 中文期刊> 《生物医学与环境科学:英文版》 >Analysis of the Electrophoretic Profiles of Prion Protein in Carcinous and Pericarcinous Lysates of Six Different Types of Cancers

Analysis of the Electrophoretic Profiles of Prion Protein in Carcinous and Pericarcinous Lysates of Six Different Types of Cancers

         

摘要

Objective To find the different electrophoretic profiles of prion protein in carcinous and individual pericarcinous tissues in lysates of gastric,colon,liver,lung,thyroid,and laryngeal cancers.Methods Sodium dodecyl sulfate polyacrylamide gel electrophoresis(SDS-PAGE)and Western blot were used to test the amounts and electrophoretic patterns of total Pr P and the tolerance of PK(protease K)digestion among six various cancer tissue types.Results A mass of Pr P signals with a large molecular weight were identified in the homogenates of peripheral tissues.The amounts and electrophoretic patterns of total Pr P did not differ significantly between carcinous and pericarcinous tissues.Pr Ps in all types of the tested cancer samples were PK sensitive but showed diversity in the tolerance of PK digestion among various tissue types.Conclusions The study revealed that the included electrophoretic patterns of carcinous and pericarcinous tissues were almost similar.Unlike Pr P-specific immunohistochemical assay,evaluation of Pr P electrophoretic patterns in the peripheral organs and tissues by Western blot does not reflect tumor malignancy.

著录项

  • 来源
    《生物医学与环境科学:英文版》 |2021年第9期|683-692|共10页
  • 作者单位

    Key Laboratory of Carcinogenesis and Translational Research(Ministry of Education);

    Head and Neck Surgery Department;

    Peking University Cancer Hospital&Institute;

    Beijing 100142;

    China;

    State Key Laboratory for Infectious Disease Prevention and Control;

    Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases(Zhejiang University);

    National Institute for Viral Disease Control and Prevention;

    Chinese Center for Disease Control and Prevention;

    Beijing 102206;

    China;

    Center for Global Public Health;

    Chinese Center for Disease Control and Prevention;

    Beijing 102206;

    China;

    Wuhan Institute of Virology;

    Chinese Academy of Science;

    Wuhan 430071;

    Hubei China;

    China Academy of Chinese Medical Sciences;

    Beijing 100700;

    China;

  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 肿瘤学;
  • 关键词

    Prion protein; Cancer; Electrophoretic profiles;

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