Protective Effects of Dimethyl-4,4'-Dimethoxy5,6,5',6'-Dimethylene Dioxybiphenyl-2,2'-Dicarboxylate on Damages of Isolated Rat Hepatocytes Induced by Carbon Tetrachloride and D-galactosamine

摘要

The rotective effect of biphenyl dimethyl dicarboxylate(DDB)on chemically induced damages was studied in isolated suspended rat hepatocytes.The experimental results showed that DDB(200μg/10^6cells) efficiently protected the hepatocytes against carbon tetrachloride(CCl4 10mmol·L^-1)and D-galactosamine(1mmol·L^1)induce damages.Membranal lipid peroxidation(malondialdehyde,MDA formation(and glutamic pyruvic transaminase(GPT) release from the hepatocytes were markedly decreased.The damage of the cell surfaces of the hepatocytes were also reduced as seen under a scanning electrom microscope(SEM). Pretreatment with DDB(300mg·kg^-1)orally ameliorated the reduction of liver glycogen and blood glucose caused by ip injection of D-galactosamine(800mg·kg^-1)in mice.When normal rats were given DDB 300mg·kg^-1 once daily for 10d,the free ribosomal protein and RNA in the liver increased significantly.These results indicate that DDB is of beneficial effects on both damaged and normal hepatocytes.