超声波辅助提取五倍子单宁酸的响应面优化实验

摘要

以水为提取溶剂，在单因素试验的基础上，利用响应面分析法优化了五倍子中单宁酸的超声波辅助提取条件。利用中心组合（Box—Behnken）实验设计原理研究液固比、超声波作用时间、超声波功率3个自变量对响应值单宁酸提取得率的影响。结果表明，单宁酸最佳超声波辅助提取条件为：5g五倍子粉末，液固比38：1（mL：g），超声波提取时间32min，超声波功率250w，在此条件下单宁酸提取得率为66．0％。%The ultrasonic-assisted extraction of tannic acid from Chinese gallnut with water as extraction agent were determined on the basis of single factor experiment using response surface method ( RSM ). According to central composite ( Box-Behnken ) design principle, the effect of three independent variables on the Yield of tannic acid, namely liquid to solid ratio, ultrasonic extraction time, and ultrasonic power was investigated. The results showed the optimum ultrasonic-assisted extraction conditions were: 5 g of Chinese gallnut powder, liquid to solid ratio 38 : 1 ( mL: g), ultrasonic extraction time 32 min, ultrasonic power 250 W. Under the optimum conditions, the extraction yield of tannic acid was 66.0 %.