组蛋白去甲基化酶KDM4B促进根尖牙乳头干细胞中成骨和成牙本质分化

摘要

目的 研究组蛋白去甲基化酶KDM4B对根尖牙乳头干细胞定向分化能力的影响.方法 人重组骨形成蛋白4(BMP4)刺激根尖牙乳头干细胞后检测KDM4B的表达；利用慢病毒转染过表达或者基因敲除KDM4B进行获得性或丧失性功能研究.通过检测碱性磷酸酶(ALP)活性、茜素红染色、钙离子定量分析研究根尖牙乳头干细胞体外成骨和成牙本质分化能力.结果 BMP4促进根尖牙乳头干细胞KDM4B的表达.基因敲除KDM4B抑制根尖牙乳头干细胞ALP活性及体外矿化能力、促进转录因子PPAR-gamma的表达.过表达KDM4B增强根尖牙乳头干细胞ALP活性和体外矿化能力.结论 组蛋白去甲基化酶KDM4B具有促进根尖牙乳头干细胞成骨和成牙本质分化的潜能.%Objective To investigate the role of histone demethylase KDM4B (lysine (K)-specific demethylase 4B) on the osteogenic and dentinogenic differentiation potential of stem cells from apical papilla (SCAPs).Methods Human recombinant bone morphogenetic protein 4 (BMP4) was used to stimulate the SCAPs.Lentivirus mediated FlagKDM4B or KDM4B ShRNA was used to over-express or knock-down the KDM4B for Gain-of-function or Loss-of-function study.ALP activity assay,Alizarin-red staining and quantitative analysis of calcium were detected to investigate the osteogenic and dentinogenic differentiation capacity of SCAPs in vitro.Results BMP4 stimulated the expression of KDM4B in SCAPs.Depletion of KDM4B inhibited ALP activity and mineralization in SCAPs,and increased the expression of PPAR-gamma.Over-expression of KDM4B enhanced ALP activity genic and mineralization in SCAPs.Conclusion Histone demethylase KDM4B promoted osteogenic and dentinogenic differentiation potential of SCAPs.