Cytotoxicity screening of Melastoma malabathricum extracts on human breast cancer cell lines in vitro

摘要

Objective:To screen the cytotoxic activity of Melasloma malabathricum（M,malubathricum）against human breast carreer cell line（MCF-7）in vitro.Methods:A three steps extraction protocol using n-hexane,chloroform and methanol as the solvents systems was carried out on leaves,stems and flowers of M.nalabathricum.Dimethyl sulfoxide was used in extracts dilution and serial dilutions were conducted to obtain five different extract concentrations（100μg/mL,50μg/mL,25μg/mL,123μg/rnL and 6.25μg/mL）.The evaluation of cell growth was determined using methylene blue assay.Results:Methanol extract from the leaves showed significant anticancer activity against MCF-7cell lines with the TC₅₀value of 7.14μg/ml while methanol and chloroform extract from the flowers exhibited a moderate activity towards MCF-7 cell line,with the IC₅₀value of 33.63μg/mL and 45.76μg/mL respectively after 72 h of treatment.Conclusions:The extracts from leaves and flowers of M.nulabatkricum showed promising anticancer activity toward human breast cancer cell lines with the lowest IC₅₀at 7.14μg/mL while the extracts from stems showed less growth inhibition activity.