Prepartion and identification of activity of anti-HPV-6b/11E1 universal ribozyme——Rz1198 in vitro

摘要

Aim: To study the preparation and cleavage activity of Rz1198 directed against HPV-6bE1 and HPV-11E1 (HPV-6b/llE1) transcripts in vitro. Methods: HPV-6b/llE1 gene fragments were cloned into T-vector trader the control ofT7 promoter, ^32P-tabeled HPV-6b/I1E1 transcripts as target-RNAs were transcribed in vitro and purified by PAGE.