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Safety evaluation of long-term vas occlusion with styrene maleic anhydride and its non-invasive reversal on accessory reproductive organs in langurs

机译:苯乙烯马来酸酐对长期输精管闭塞的安全性评价及其对叶猴附属生殖器官的无创逆转

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摘要

Aim: To evaluate the safety of the long term vas occlusion with styrene maleic anhydride (SMA) and its non-invasive reversal at the level of accessory reproductive glands (ARGs) in langurs. Methods: The morphology of seminal vesicle and ventral prostate was evaluated by light as well as transmission electron microscopy. Serum clinical chemistry and urine albumin were evaluated in an autoanalyzer using reagent kits. Fructose, acid phosphatase and zinc in the seminal plasma were evaluated spectrophotomet-ically according to the WHO manual. Serum testosterone,prostate specific antigen and sperm antibodies were evaluated by enzyme-linked immunosorbent assays (ELISA)using reagent kits and hematology was estimated according to standard procedures. Results: The morphological features and secretory activity of the seminal vesicle and prostate were normal as evidenced by the presence of welldeveloped mitochondria, rough endoplasmic reticulum, Golgi bodies, secretory granules and normal nuclear characteristics throughout the course of investigation. Serum testosterone and prostate specific antigen remained unaltered and serum antisperm antibodies level presented negative titres. Urine albumin was nil. Total red blood corpuscles (RBC), white blood corpuscles (WBC), hemoglobin (Hb) and red cell indices, serum protein, glucose, cholesterol,creatinine, creatine kinase (CK), serum glutamate oxalate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT), lactate dehydrogenase (LDH), bilirubin, urea, triglycerides and high-density lipoprotein (HDL) did not show appreciable changes following vas occlusion and after its non-invasive reversal. Although fructose, acid phosphatase (ACP) and zinc in the seminal plasma showed a significant reduction following vas occlusion, it could not be related to the morphology of seminal vesicle and prostate. Conclusion: SMA vas occlusion and its non-invasive reversal do not damage the accessory reproductive organs.
机译:目的:在叶猴的辅助生殖腺(ARG)水平上,评估长期使用苯乙烯马来酸酐(SMA)进行输精管闭塞的安全性及其无创性逆转。方法:通过光镜和透射电镜观察精囊和腹侧前列腺的形态。使用试剂盒在自动分析仪中评估血清临床化学和尿白蛋白。根据WHO手册,分光光度法评估了精浆中的果糖,酸性磷酸酶和锌。使用试剂盒通过酶联免疫吸附测定(ELISA)评估血清睾丸激素,前列腺特异性抗原和精子抗体,并根据标准程序评估血液学。结果:在整个研究过程中,精子的线粒体,粗面的内质网,高尔基体,分泌颗粒和正常的核特征证实了精囊和前列腺的形态特征和分泌活性正常。血清睾丸激素和前列腺特异抗原保持不变,血清抗精子抗体水平呈阴性滴度。尿白蛋白为零。总红血球(RBC),白血球(WBC),血红蛋白(Hb)和红细胞指数,血清蛋白,葡萄糖,胆固醇,肌酐,肌酸激酶(CK),血清谷氨酸草酸转氨酶(SGOT),血清谷氨酸丙酮酸转氨酶(SGPT),乳酸脱氢酶(LDH),胆红素,尿素,甘油三酸酯和高密度脂蛋白(HDL)在输精管闭塞后和无创逆转后均未显示明显变化。尽管精子血浆中的果糖,酸性磷酸酶(ACP)和锌在输精管闭塞后显示出明显减少,但与精囊和前列腺的形态无关。结论:SMA血管闭塞及其无创性逆转不会损害附属生殖器官。

著录项

  • 来源
    《亚洲男性学杂志(英文版)》 |2005年第002期|195-204|共10页
  • 作者单位

    Reproductive Physiology Section, Department of Zoology, University of Rajasthan, Jaipur-302 004, India;

    Reproductive Physiology Section, Department of Zoology, University of Rajasthan, Jaipur-302 004, India;

    Reproductive Physiology Section, Department of Zoology, University of Rajasthan, Jaipur-302 004, India;

    Reproductive Physiology Section, Department of Zoology, University of Rajasthan, Jaipur-302 004, India;

    Reproductive Physiology Section, Department of Zoology, University of Rajasthan, Jaipur-302 004, India;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 chi
  • 中图分类 R3;
  • 关键词

    langurs; male contraception; poly styrene maleic anhydride;

    机译:Langurs;男性避孕;聚苯乙烯马来酸酐;
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