Rana maoershanensis found in Mt.Maoershan in Guangxi,China was reported as a new species in 2007,but there was no molecular data for this frog.The partial sequences(543 bp) of 16S rRNA gene from 12 specimens of 3 brown frog species(Rana hanluica,R.maoershanensis and R.chensinensis) were analyzed with 17 specimens of 9 species from GenBank.The nucleotide sequence divergence between R.maoershanensis and the other brown frog species were 4.5%-6.5%,with 22-30 nucleotide substitutions at this locus.The phylogenetic relationships based on MP,ML,and Bayesian inference indicate that the brown frogs from Southern China were diverged into three groups(Clades A,B and C).R.maoershanensis was clustered together a well-supported subclade(B-1).It is suggested that R.maoershanensis is a valid species.
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