Effects of 8-Hydroxyisocapnolactone-2-3-diol and friedelin on mast cell degranulation

摘要

Objective: To investigate the effects of friedelin(terpenoid) and 8-hydroxyisocapnolactone-2-3-diol(coumarin) with concentration 10 mM, 30 m M, and 100 mM on inhibiting mast cells(MCs) degranulation.Methods: The investigation was performed in vitro by administering each compound into rat peritoneal MCs and rat basophilic leukemia-2 H3 cells followed by activation with50 mg/m L of compound 48/80 or 1 m M of ionomycin. The concentration of histamine released from each group was measured by a high-performance liquid chromatographyfluorometry system with post-column derivatization using o-phthalaldehyde.Results: 8-Hydroxyisocapnolactone-2-3-diol inhibited degranulation of compound 48/80 activated-rat peritoneal MCs with the histamine release percentages of 74.57%,72.21% and 51.79% when the 10 mM, 30 m M and 100 mM concentrations were used,respectively. Where as about 81% histamine was released by the control group.Degranulation inhibition ability was also observed in ionomycin-activated rat basophilic leukemia-2 H3 cells. In contrast, friedelin failed to inhibit degranulation in either cell type.The inhibition of 8-hydroxyisocapnolactone-2-3-diol was not related to the depletion of histamine synthesis as implied by the total histamine measurement.Conclusions: These results exhibit the promising of 8-hydroxyisocapnolactone-2-3-diol is a potential parent structure for developing a MCs stabilizer.