Evaluation of bioefficacy of毩-cypermethrin in long lasting impregnated net (Interceptor®) using analytical method

摘要

Objective:To evaluate relationship betweenα-cypermethrin residues in Interceptor® nets by using HPTLC methods and mortality percentage in standardWHO's method "cone test" after repeated washings.Methods: Interceptor® containing 毩-cypermethrin was provided byBASF Company. The washing procedure and bioassay tests were carried out according to theWHO-recommended methods. Malaria vector, An. stephensi was exposed to impregnated bed net for three minutes and then mortality measured after24 hour recovery period. Chemical analysis was carried out in chemical laboratory by using high performance thin layer chromatography method.Results:Result of cone bioassay method showed that washing reduced the efficacy of Interceptor® bed net from100% in unwashed nets to15% at20 washes. After 20 washes, nets contain (61.2±2.8)mg/m2resulting15%mortality in cone test. Killing activity was reduced when nets were washed.Conclusions:Results will provide an essential clue for monitoring and evaluation of bioefficacy of any long lasting impregnated bed net for quality control. Findings of this study will be useful forWHO, local investigators and people who wish to use pyrethroid-impregnated bed nets for malaria vector control.