首页> 中文期刊> 《亚太热带医药杂志(英文版)》 >Intra-species sequence variability in 28s rRNA gene of Oesophagostomum venulosum isolated from goats of West Bengal, India

Intra-species sequence variability in 28s rRNA gene of Oesophagostomum venulosum isolated from goats of West Bengal, India

         

摘要

Objective:To identify genotypes of Oesophagostmum venulosum(O. venulosum) prevailing in West Bengal, India by comparing variation of nucleotide sequences among 28S rRNA. Methods:PCR amplification of partial segment of 28 S rRNA sequence and analysis of sequence amplified product by single strand conformation polymorphism (SSCP). Results:Two distinct conformers among male and female parasites were identified by PCR-SSCP analysis. Sequence analysis among conformers revealed the presence of five single nucleotide polymorphisms (SNP) in codon 64, 66, 86, 125 and 146. Secondary RNA prediction structure showed that out of 5 SNPs, 4 occurred at interior loop of RNA which confirmed evolutionary changes among isolates prevailing in this region. Conclusions:SNPs occured in different isolates of O. venulosum might influence critical changes in rRNA folding pattern which influence evolutionary changes among isolates.

著录项

  • 来源
    《亚太热带医药杂志(英文版)》 |2010年第007期|515-518|共4页
  • 作者单位

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

    National Research Centre on Yak, Dirang, Arunachal Pradesh;

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

    Eastern Regional Station of Indian Veterinary Research Institute, 37 Belgachia Road, Kolkata-37, West Bengal, India;

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