Isolation and characterization of halophilic Bacillussp. BS3 able to produce pharmacologically important biosurfactants

摘要

Objective:To characterize the pharmacological importance of biosurfactants isolated from halophilicBacillus spBS3.Methods:HalophilicBacillus sp.BS3 was isolated from solar salt works, identified by16S rRNA sequencing and was used for screening their biosurfactant production.Characters of the biosurfactant and their anticancer activity were analyzed and performed in mammary epithelial carcinoma cell at different concentrations.Results:The biosurfactant were characterized byTLC,FTIR andGC-MS analysis and identified as lipopeptide type.GC-MS analysis revealed that, the biosurfactant had various compounds including13-Docosenamide,(Z);Mannosamine,9- andN,N,N',N'-tetramethyl.Surprisingly the antiviral activity was found against shrimp white spot syndrome virus(WSSV) by suppressing the viral replication and significantly raised shrimp survival(P<0.01).Anticancer activity performed in the mammary epithelial carcinoma cell at different concentrations of biosurfactants, among the various concentrations of biosurfactants such as0.00025,0.0025,0.025,0.25 and2.5μg, the 0.25 μg concentration suppressed the cells significantly(P<0.05) to24.8%.Conclusions:Based on the findings, the present study concluded that, there is a possibility to develop eco-friendly antimicrobial and anticancer drugs from the extremophilic origin.