Expression of soluble vascular endothelial growth factor receptor-1 and placental growth factor in fetal growth restriction cases and intervention effect of tetramethylpyrazine

摘要

Objective:To investigate the expression of soluble vascular endothelial growth factor receptor-1 (sFlt-1) and placental growth factor(PLGF) in the fetal growth restriction(FGR) cases and the intervention mechanism of tetramethylpyrazine.Methods:A total of60 fetal growth restriction cases that admitted to our hospital were randomly divided into ligustrazine intervention group (groupA) and nutritional support group(groupB).A total of50 healthy pregnant women were also enrolled as control group(groupC).Expression level of maternal serum sFlt1,PLGF and fetal growth parameters includingHC,AC,FL,BPD,EFW as well as placentaPLGF, sFlt-1 mRNA expression were recorded and compared among the three groups.A total of15SD rats were selected and were divided into three groups,TMP group, alcohol and tobacco group and blank control group.Three groups of rats were dissected on the twentieth day of gestation.Result:Expression level of sFlt-1 andPLGF in groupA was not significantly different from that of group C(P>0.05); but significant difference inSFlt1 andPLGF expression level was observed between groupC and groupB(P<0.05).Before treatment,HC,AC,FL,BPD andEFW of groupA and groupB were significant lower than those of groupC, but after treatment, those parameters in groupA were significantly improved(P<0.05).In the animal experiment there was no significant difference in sFlt-1 between treatment group andFGR group without treatment or control group (P>0.05).There was significant difference inPLGF betweenFGR group with treatment andFGR group without treatment or control group(P<0.01).Conclusions:PLGF level is decreased and sFlt-1 increased in patients suffered from fetal growth restriction, andFGR rats show increased sFlt-1 and decreasedPLGF, thus they can be indicator of the fetal growth restriction.Ligustrazine can effectively improve sFlt-1,PLGF expression level in fetal growth restriction cases, which can be used as treatment forFGR.