Protective effect of TMP on pancreas function of acute pancreatitis rats

摘要

Objective:To explore the protective effect and mechanism of Tetramethyl Pyrazine(TMP)on the pancreas function of acute pancreatitis rats.Methods:A total of 75 SD rats were randomly divided into three groups(A,B,C) with 25 rats in each group.Group A served as sham operation group.In the groups B and C,AP model was prepared as by injecting taurocholic acid sodium.Group B was model group.After modeling,rats were administrated by intraperitoneal injection of normal saline.Group C was TMP treatment group,which was administrated by intraperitoneal injection of 0.6% TMP after modeling.The rat blood specimens in each group were collected with 1 mL/100 g solution after modeling of 2,6.12 and 24 h.Levels of amylase(AMS).blood urea nitrogen(BUN),creatinine(CR).TNF-α and IL-6 were detected,and 5 rats were sacrificed.Histopathological examination was performed in he pancreatic tissue specimens of each group to observe pancreatic tissue damage.Results:After modeling in each time point.AMS,BUN.CR.TNF-α and IL-6 in groups B and C were significantly higher than that of in group A(P<0.05).After modeling of 2 h.AMS.BUN and CR in group B increased significantly and reached the peak value at6 h.After modeling of 12 h.scrum level of TNF-α and IL-6 were significantly lower than that of in control group,while after 24 h of modeling,serum level of AMS.BUN,CR,TNF-α and IL-6 were significantly lower than that of in control group(P<0.05).The histological observation showed that pancreatic tissue in rats of group A was normal without damage lesions.Massive bleeding,necrosis and serious injury were visible in pancreatic tissue of group B.The rut pancreatic tissue was bleeding in group C with small pieces of necrotic lesions.The degree of inflammatory cell infiltration was lower than group B.and the degree of injury was significantly lower than group B.Conclusions:TMP can significantly decrease the serum level of TNF- α and IL-6 in AP rats,inhibits inflammatory response of AP.and has significant protective effect on pancreatic tissue and function in AP rats.