Chloride channel involved in the regulation of curcumin-induced apoptosis of human breast cancer cells

摘要

Objective:To investigate the role of CIC-3 chloride channel in the proliferation of breast cancer cell line Mcf-7 treated with curcumin and its specific mechanism.Methods:MTr assay was used to detect the effect of chloride channel blocker (DIDS) and curcumin on Mcf-7 and human normal cell viability.Patch-clamp technique was used to determine the current density before and after drug treatment.Apoptosis assay by flow cytometry was performed for further examination of cell apoptosis.Results:Curcumin had toxicity on Mcf-7 and HUVEC cells and DIDS reduced the survival rate of Mcf-7 cells by inhibiting proliferation.Curcumin could activate the chloride ion current on MCF-7 cell membrane,which would be inhibited by DIDS.Finally,curcumin in low concentration combined with DIDS could significantly promote the MCF-7 cells apoptosis.Conclusions:Our results suggest that CIC-3 protein is involved in the regulation of curcumin induced proliferation inhibiting in breast cancer cells through inducing cell apoptosis.ClC-3 may be a potential target of tumor therapy.