Objective: To Assessment of pharmacognostic and phytochemical parameters of root of Thespesiapopulnea (L.) soland. Methods: Macroscopical, miscroscopical, physico-chemical evaluation, florescence analysis, behavior of root powder and preliminary phytochemical analysis, quantitative estimation of phytoconstituents were determined of various extracts of T. populnea.Results: microscopic study shows the general characteristic of root with presence of periderm, cortex, xylem and phloem region, abundant starch grains. Physico-chemical investigation shows the total ash, acid insoluble ash, water soluble ash and sulphated ash values were 10.59依0.02 % w/w, 1.02依0.03 % w/w, 2.72依0.03 % w/w and 8.56依0.02 % w/w respectively. However, the aqueous soluble, alcohol soluble extractives and moisture content were found to be 14.23依0.46 % w/w, 10.54依0.23% w/w and 4.60依0.01% w/w respectively. The preliminary phytochemical assessment revealed the presence of glycosides, steroid/triterpenoids, flavonoids, tannins, phenolic compounds saponins, carbohydrates and proteins. Total phenolic and flavonoids content of root was 0.271%and 0.344% respectively. Behavior of root powder with various chemicals confirmed the presence of phytoconstituents. Conclusions: The pharmacognostic and phytochemical assessment of T. populnea may helpful towards founding for quality, purity and sample identification and standardization.
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