Effect of different concentration of fish oil in skim milk-egg yolk extenders on post-thawed semen qualities of Kalang swamp buffalo bull

摘要

Objective:To explore the effect of fish oil at different concentrations on post-thawed semen of Kalang swamp buffalo.Methods: A total of 4 Kalang swamp buffalo bulls with 3-5 years of age and weighed about 340-360 kg were slected. Semen was regularly collected from these buffalo bulls once a week by an artificial vagina. Fish oil was supplementary at the dosages of 0 mg (control), 50 mg, 100 mg, 150 mg, and 200 mg to the extender (skim milk-egg yolk). Fresh, pre-freezing and frozen semen were thawed at 37℃ and evaluated for motility, viability, morphology, and plasma integrity of membrane.Results: The study results indicated that before freezing, supplementation of fish oil at the dose of 150 mg in the extender had significantly motility. And a significant (P<0.05) increase was observed in viability and motility of post-thawed semen at the dose of 150 mg fish oil, which was in difference with other treatment groups.Conclusions: Addition of 150 mg fish oil in the extender could be positive for the enhancement of the quality of post-thawed semen of Kalang swamp buffaloes.