超声波处理对生物破乳菌Alcaligenes sp.S-XJ-1破乳性能影响研究

摘要

To investigate the relationship between the cell integrity and demulsifying ability, the influence of ultrasonic treatment on demulsifying strain of Alcaligenes sp. S-XJ-1 was studied. The demulsifying strain was isolated from the contaminated soil near an oil production well in Karamay oil field of Xinjiang. The results showed that the strain treated by ultrasonic cell disruption system ( power intensity, 0. 2 W/mL) had a rougher cell surface and flat shape. The cell surface hydrophobicity was changed and the highest cell surface hydrophobicity achieved 95 % treated by 5 min. After treated for 30 min by ultrasonic, the emulsion breaking ratio of the demulsifying strain dropped to 55%. Also,there was a negative relationship(R2 =0.884) between DNA concentration and emultion breaking ratio. The study also found that cells accelerated the coalescence process by absorption bridging effect which contributed to its higher demulsifying efficiency.%以新疆克拉玛依油田某采油井附近长期受石油污染的土壤中筛选出的生物破乳菌Alcaligenes sp.S-XJ-1为研究对象,考察超声波处理对生物破乳菌菌体性质和破乳性能的影响.结果表明,超声波处理(0.2 W/mL)后菌体表面变粗糙,部分细胞呈现凹陷或扁平状.短时间超声波处理(0～5 min)使菌体颗粒粒径迅速降低,菌体细胞表面疏水性提高,最大值达到95％；超声波处理30 min后,其对W/O乳状液的破乳率降至55％.研究还发现,细胞破碎程度与破乳率呈现负相关(R2=0.884),推测菌体细胞通过吸附架桥作用加速液滴聚集聚并过程,从而提高破乳速率和破乳率.