Radiation-induced DNA damages include base deletion,single-strand break and double-strand break(DSB).Among them,DSB is the most harmful,which could leads to gene mutation,cell apoptosis or carcinogenesis[1].Histone H2AX was phosphorylated(-H2AX)at Ser-139 rapidly after DSB damage.Quantitative analysis of-H2AX focus by fluorescence microscopy has become the popular method for DSB detection that each break has been found to correspond to one-H2AX focus.-H2AX plays an important role in damage signal cascading that the modification of chromatin improves DNA accessibility and leads to the recruitment and accumulation of specific DNA damage response(DDR)proteins at the DNA end[2,3].
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