3-35 ISSR Assay of M2 Mutants of Lotus japonicus Induced by Carbon Ion Beam Irradiations

摘要

To investigate the DNA polymorphism of M2 plants of Lotus japonicus induced by heavy ion beam, dry seeds were irradiated by carbon ion beams accelerated by Heavy Ion Research Facility in Lanzhou (HIRFL). Inter simple sequence repeat (ISSR) assay were utilized to explore the DNA variation of M2 plants irradiated by 400 Gy carbon ion beams. ISSR technique is an effective molecular marker method based on polymerase chain reaction amplification of template DNA by a single primer with 1626 bp[1]. ISSR can detect the differences of genetic structure of various life materials rapidly.