Probiotic Cocktails Alleviate Heat-induced Intestinal Barrier Dysfunction and Oxidative Stress in Broiler Chickens

摘要

[Objective]To investigate the effect of probiotic cocktails on growth performance,intestinal barrier function and antioxidant status in heat-stressed broilers.[Methods]One thousand 1-day-old broilers were randomly divided into five groups:the thermoneutral(TN),heat stress(HS),heat stress with antibiotic(HS-AB),heat stress with probiotic cocktail 1(HS-P1),and heat stress with probiotic cocktail 2(HS-P2)groups.The broilers in TN and HS groups received a diet without supplements,and those in HS-AB,HS-P1 and HS-P2 groups were fed a diet supplemented with chlortetracycline(300 g/t)or probiotic cocktail 1 or 2(100 g/t),respectively.[Results]The results showed that heat stress reduced growth performance and increased inflammatory factors,NF-κB and Toll-like receptors in jejunal mucosa coinciding with higher expression of jejunal tight junction proteins,mucin and heat stress protein mRNA.[Conclusion]Dietary probiotic cocktails had protective effects against the heat stress-induced changes in the intestine,with increased serum antioxidant capacity and growth performance of broilers.The effect of these cocktails was similar to that of chlortetracycline.