Detection of Antibodies against Toxoplasma gondii by ELISA with Recombinant Microneme Protein 3

摘要

[Objective] To develop a new method for serodiagnosis of swine toxoplasmosis.[Method] With the purified recombinant microneme protein 3 (rMIC3) as coating antigens,an indirect ELISA was developed for detection of antibodies against Toxoplasma gondii.[Result] The optimal working concentration of rMIC3 was 3.40 μg/ml,and the optimal degree of dilution of sera was 1:160.Cross-reaction was not observed between the Toxoplasma gondii-positive sera and the positive sera against classical swine fever virus or some other pathogens.The developed ELISA had 92.56% coincidence rate with latex agglutination test.[Conclusion] The developed ELISA is sensitive,rapid,specific and reproducible,and thus it can be applied in serodiagnosis and seroprevalence investigation of swine toxoplasmosis.