Screening of Serum Dilution and Determination of Normal Range of Antibodies against Rabies Viruses in Dog Sera

摘要

[Objective] To screen a suitable serum dilution and determine the normal range of antibodies against rabies viruses(RV) in dog serum.[Method] A sensitive,specific and suitable serum dilution was screened.A total of 812 dog serum samples were collected from Changchun,Nanning and Liuzhou regions,and then they were diluted with above screened serum dilution and evaluated by indirect enzyme-linked immunosorbent assay(ELISA).The positive and negative standard dog sera from the World Organization for Animal Health(OIE) were used as controls.The serum samples with different A450 were randomly selected and the anti-RV serum titers were detected by rapid fluorescent focus inhibition test(RFFIT).According to the A450 of dog sera,the normal A450 range of negative serum and 95% confidence intervals were calculated with SPSS10.0 software,and the regression equation of OIE standards was established.[Result] The screened serum dilution was sensitive and specific;at least 756 negative serum samples were obtained;the normal range and 95% confidence interval of negative serum were 0.127 3 ± 0.059 8 and 0.078 1-0.172 3,respectively;and the regression equation was A450=1.139 serum titer(IU)+0.470.[Conclusion] These results lay a foundation for the controlling of dog rabies endemic and the development of ELISA kits of dog antibodies against RV.