Choline is a necessary nutrient for dairy cows and contributes to milk yield,but most of dietary choline can be degraded by the microorganisms in rumen.Rumen protected choline(RPC) has been developed to protect choline from degradation.To screen stable RPC,choline chloride was coated with acrylic resin no.IV,chitosan and the mixture containing the two lagging covers by fluidized bed coating technology,respectively.Their stability was evaluated in vitro and in situ.The results showed that release rate of RPC coated with acrylic resin no.IV and chitosan was the lowest in rumen and pH 6.6 buffer.And its effective protection rate reached 62.04% in rumen.Therefore,RPC coated with acrylic resin no.IV and chitosan has good stability and protection efficacy in rumen,and it can be used in practice to supply effective choline for the ruminant.
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