[Objective] To optimize solid state fermentation conditions of rapeseed meal and thus to reduce glucosinolates and neutral detergent fibers by mixed cultures of Aspergillus oryzae and Trichoderma viride.[Method] The optimal fermentation conditions were determined by single factor test and orthogonal design.[Result] The optimum fermentation conditions are as following:inoculum weight ratio(Aspergillus oryzae vs Trichoderma viride),1:1;inoculum size,30%;water content,40%;fermentation time,96 h;and fermentation temperature,30 °C.Under these conditions,glucosinolates were reduced by 90.71% and neutral detergent fibers were degraded by 20.65%.[Conclusion] In laboratory,solid state fermentation with Aspergillus oryzae and Trichoderma viride can be used to produce high-quality rapeseed meal.
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