[Objective] To obtain ovine oocytes 2-dimensional gel electrophoresis (2-DE) maps. [Method] Ovine oocytes were solubilized in lysis buffer, and protein concentrations were measured using the method of Bradford and Ramagli. Then the samples were subjected to two-dimensional polyacrylamide gel electrophoresis. The 2-DE maps of ovine oocytes were analyzed through PDQuest 8.0. [Result] The Ramagli method could reflect concentration of oocyte protein more actually and could optimize the quantity of samples to get a 2-DE map with higher quality. The protein concentration measured by the Bradford method was higher than its actual value. [Conclusion] Each 2-DE map loading 700 oocytes is more reasonable.
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