Cloning Sequencing and Bioinformatics Analysis of GRX2 and TRX2 from Puerpiao chicken and Tengchongxue chicken

摘要

[Objective] The study was to explore whether glutaredoxin( GRX2) and thioredoxin( TRX2) genes vary between Puerpiao chicken and Tengchongxue chicken in Yunnan Province. [Methods] Using Sanhuang chicken as the control,the GRX2 and TRX2 genes were cloned from skin tissues of Puerpiao chicken and Tengchongxue chicken and then subjected to bioinformatics analysis. [Results] The sequencing results of both the GRX2 and TRX2 genes were completely consistent among Puerpiao chicken,Tengchongxue chicken and Sanhuang chicken. And the sequences of GRX2 and TRX2 genes share a 100% homology with the results accessed in Gen Bank,and no any mutation was observed. [Conclusion]The result provides scientific basis for investigating the antioxidant capacity of different chickens and utilizing elite local chicken resources.