Effect of Different Concentrations of Lipopolysac- charide on Expression of NF-KB and LAP in Mammary Epithelial Cells of Dairy Cow

摘要

The paper was to study the effects of different concentrations of lipopolysaccharide(LPS) on expression of nuclear factor Kappa B(NF-κB) and lingual antimicrobial peptide(LAP) gene in mammary epithelial cells of dairy cow.The mammary epithelial cells of dairy cow were stimulated by different concentrations(50,100,200,400 and 800 ng/mL) of LPS.The total RNA of cells was extracted after stimulation for 2,4,8,16,24,48 and 72 h,respectively,and the mRNA expression levels of NF-κB P65 and LAP were evaluated by real-time quantitative PCR.The results showed that the expression of NF-κB P65 and LAP mRNA treated with 400 ng / mL LPS for 72 h were the highest compared to the control group(P<0.01).The result confirmed that the expression activity of NF-κB was enhanced in inflammatory effects of mammary epithelial cells induced by LPS,which regulated the expression of defense gene LAP,with certain dose and time effects.