Comparative Analysis of Microsatellite DNA Polymorphism in Gold Grass Carp and Four Grass Carp Populations from China

摘要

[Objectives]To analyze the genetic structure and genetic diversity of gold grass carp population.[Methods]A total of 15 microsatellite markers were used to examine gold grass carp population and four grass carp populations[Yuanjiang(YJ),Ningxiang(NX),Honghu(HH)and Xijiang(XJ)]from the Yangtze River and the Pearl River of China.[Results]All the 15 microsatellite loci were highly polymorphic,with polymorphism information content (PIC) of 0.763-0.939.The expected heterozygosity(H_E)of gold grass carp population was 0.662,lower than those of the four Chinese grass carp populations(H_E=0.85-0.885).The genetic differentiation index(F_(ST))between gold grass carp population and YJ,NX,HH,XJ populations were 0.157 2,0.129 5,0.147 5 and0.114 4,respectively.This suggests that gold grass carp population is highly differentiated from YJ grass carp population(0.15